Variability in feed intake the first days following weaning impacts gastrointestinal tract development, feeding patterns, and growth performance in nursery pigs.

The present study investigated the effects of voluntary feed intake (FI) the first days after weaning on gastrointestinal development and protein fermentation the first week after weaning and growth performance and feeding patterns during the nursery phase. A total of 144 mixed-sex weaned pigs (24 ±â€…2 d old; 7.2 ±â€…0.8 kg body weight [BW]) were allocated to 12 pens with 12 pigs/pen. Each pen was equipped with an electronic feeding station for monitoring individual FI during a 40-d study. Pigs were classified based on their cumulative FI over the initial 3 d after weaning (FId1-3) being above or below their pen median FId1-3 (high = 919 ±â€…244 g or low = 507 ±â€…222 g FId1-3). Similarly, weaning BW classes (BW0; high = 7.72 ±â€…0.59 kg or low = 6.62 ±â€…0.88 kg BW) were created to study interactions with FId1-3. Two female pigs with either a high or a low FId1-3 per pen (n = 24) were selected for sampling at d6 and were used to study gastrointestinal development and fermentation products in the small intestine. Feeding patterns per day, FI, and growth performance were measured individually. Low FId1-3 pigs had lower (P < 0.05) daily FI during d0 to d8, d8 to d15, and d22 to d28, BW on d15, d22, d29, and d40, and average daily gain during d0 to d8, d22 to d29, and d29 to d40 compared to high FId1-3. High FId1-3 pigs increased (P < 0.05) the number of visits to the feeder between d1 to d13 and d31 to d35, and the time spent per visit only for d1 to d4 (P < 0.05). The daily rate of FI (g/min) was higher (P < 0.05) for High FId1-3 pigs on d6, d8, d9, and d10, and again several days later (d20 to d39). In addition, the high FId1-3 × high BW0 interaction improved daily FI during d18 to d40 compared to low FId1-3 × low BW0 class (P < 0.05). For the sampling on d6, low FId1-3 pigs had a lighter small intestine, colon, and pancreas, and reduced villi length, smaller villi surface area, and a lower number of goblet cells size in jejunum (P < 0.05), while concentrations of lactic acid, histamine, and cadaverine in small intestinal content were increased (P < 0.05). In conclusion, pigs with high FId1-3 became faster eaters with higher FI and growth rates toward the second half of the nursery, which was similar and additive for pigs with higher weaning BW. High FId1-3 was also associated with greater development of the gastrointestinal tract and a reduced protein fermentation 1-wk after weaning.

Poor adaptation to solid feed after weaning is often associated with a reduced digestive function and growth in nursery pigs. The reasons driving an early acceptance of feed and its consequences are still largely unknown. We investigated the effects of high and low feed intake between d1-3 after weaning on gastrointestinal development and morphometrics 1-wk after weaning and growth performance and feeding patterns in the nursery phase. The results showed that pigs with a high initial feed intake (increased number of visits to the feeder and time spent per visit early after weaning), consumed feed faster throughout the nursery resulting in higher intakes early and late in the nursery but not for the intermediate period. Higher weaning body weight was also associated with improved feed intake and growth from d17 onwards, which was an additional but independent effect of the early feed intake effect. Besides, pigs with high feed intake between d1 and d3 after weaning had heavier empty gastrointestinal organs, improved intestinal wall morphometrics, and reduced protein fermentation in the small intestine 1-wk after weaning.

Review: The amazing gain-to-feed ratio of newly weaned piglets: sign of efficiency or deficiency?

Shortly after weaning, piglets generally eat dry feed poorly; but nevertheless, a phenomenal gain-to-feed ratio is achieved as they gain about as much weight as they eat (150-200 g/d). The high gain-to-feed ratio, though, cannot be explained by their nutrient intake or nutrient repartitioning. Analyses based on tissue composition and bio-electrical impedance data showed that newly weaned piglets lose fat, maintain protein, and gain large amounts of water because of edema. This edema, which may well contribute up to one kg of BW, seems to be triggered by refeeding syndrome. Refeeding syndrome in adult humans occurs when subjects fast for an extended period of time (weeks) that results in downshifts in metabolic activity and concomitant shedding of phosphate (PO4), magnesium (Mg), and potassium (K) in urine. If food is abruptly reintroduced, thus, resulting in strong insulin spikes, metabolism is triggered but hampered by a lack of PO4, Mg, K, and thiamine, causing hypophosphatemia, metabolic stress, and edema. In piglets, the same process appears to happen immediately after weaning but in hours rather than weeks, possibly linked to their high metabolic rate. Refeeding syndrome can be lethal in humans but does not appear to be directly lethal in piglets. Our attempts to prevent it through altered diet composition and/or controlled feeding programs have not resulted in better performance at the end of the nursery phase. A practical ramification of weaning-induced edema is that growth and gain-to-feed ratio data immediately after weaning should be interpreted with caution. In addition, diets arguably should be formulated to not strongly trigger insulin release, while high lysine levels are not needed as the gain is not based on protein accretion.

A blend of medium-chain fatty acids, butyrate, organic acids, and a phenolic compound accelerates microbial maturation in newly weaned piglets.

Inclusion of additive blends is a common dietary strategy to manage post-weaning diarrhea and performance in piglets. However, there is limited mechanistic data on how these additives improve outcomes during this period. To evaluate the effects of Presan FX (MCOA) on the intestinal microbiota and metabolome, diets with or without 0.2% MCOA were compared. Pigs fed MCOA showed improved whole-body metabolism 7 days post-weaning, with decreased (P < 0.05) creatine, creatinine and ß-hydroxybutyrate. Alterations in bile-associated metabolites and cholic acid were also observed at the same time-point (P < 0.05), suggesting MCOA increased bile acid production and secretion. Increased cholic acid was accompanied by increased tryptophan metabolites including indole-3-propionic acid (IPA) in systemic circulation (P = 0.004). An accompanying tendency toward increased Lactobacillus sp. in the small intestine was observed (P = 0.05). Many lactobacilli have bile acid tolerance mechanisms and contribute to production of IPA, suggesting increased bile acid production resulted in increased abundance of lactobacilli capable of tryptophan fermentation. Tryptophan metabolism is associated with the mature pig microbiota and many tryptophan metabolites such as IPA are considered beneficial to gut barrier function. In conclusion, MCOA may help maintain tissue metabolism and aid in microbiota re-assembly through bile acid production and secretion.

Impact of L-Arginine and L-Glutamine supplementation on growth performance and immune status in weanling pigs challenged with Escherichia coli F4.

Arginine (ARG) and Glutamine (GLN) have been reported to play significant roles in protein metabolism, immunity, and intestinal health in weanling pigs. The present study investigated the independent and interactive effect of supplementing ARG and GLN on pigs immune status and growth performance following an Escherichia coli F4 challenge. A total of 240 mixed-sex pigs (24 ±â€…2 d old; 7.3 ±â€…0.1 kg BW) were used in a 42-d experiment after selection for E. coli F4 susceptibility. The pigs were group-housed (3 pigs per pen), and pens were randomly assigned to five experimental treatments (N = 16 pens per treatment). Experimental treatments were: 1) a wheat-barley-soybean meal-based basal diet (CTRL), 2) a basal diet with 2500 mg/kg zinc oxide (ZnO), 3) a basal diet + 0.5% Glutamine (0.5% GLN), 4) basal diet + 0.5% Arginine (0.5% ARG), and 5) basal diet with 0.5% Glutamine + 0.5% Arginine (0.5% GLN + ARG). All Pigs were inoculated with E. coli F4 on days 7, 8, and 9 post-weaning. Rectal swabs were taken from each pig and plated on blood agar plates for E. coli F4 presence. Blood and fecal samples were taken to determine the acute phase response and selected fecal biomarkers for the immune response. Growth performance and fecal scores were recorded. Fecal swabs resulted in no positive pig for E. coli F4 before inoculation and 73.3% positive postinoculation. Diarrhea incidence during days 7 to 14 was significantly lower for the ZnO treatment (P < 0.05). The haptoglobin level on day 3 was lower than days 10 and 20, irrespective of treatment (P < 0.05). The albumin level was lower on day 20 compared to days 3 and 10 (P < 0.05). There was no treatment effect on albumin levels regardless of sampling day (P > 0.05). The PigMAP was lowest on day 3 and highest on day 10 (P < 0.05). We did not observe significant treatment differences (P > 0.05) in myeloperoxidase and calprotectin. Pancreatitis-associated protein was higher in the ZnO (P = 0.001) treatment than in the other treatments. Fecal IgA tended (P = 0.10) to be higher in the ZnO and 0.5% ARG treatments. There were no performance differences, except during days 0 to 7, where the ZnO treatment was lower in average daily gain and average daily feed intake (P < 0.001), while feed efficiency (G:F) FE was similar across treatments. In summary, no improved performance was observed with either ARG, glutamate, or both. The immune response results showed that the E. coli F4 challenge may have exacerbated the acute phase response; hence, the benefits of dietary treatments did not go beyond immune repair and reduction in inflammation.

The supplementation of functional amino acids such as arginine and glutamine has been reported to improve growth performance in weanling pigs. However, during periods of enteric Escherichia coli challenge, results have been inconsistent. Here, we investigated whether independent or combined arginine and glutamine supplementation could improve performance and immune response under an E. coli F4 challenge. The results showed no performance improvements but an improvement in the acute phase response and immune response status. Thus, the benefit of supplemental arginine and glutamine may be prioritized towards building an immune response rather than growth under conditions of an enteric immune challenge.

The effect of supplementation of essential amino acid combinations in a low crude protein diet on growth performance in weanling pigs.

The present study investigated the impact of providing different supplemental essential amino acids (EAA) in a low crude protein (CP) diet on growth performance in weanling pigs. A total of 324 mixed-sex 24-d weaned piglets (initial BW 6.9 ± 0.34 kg) were used in a 27-d growth trial with six dietary treatments immediately post-weaning. The first two treatments were a control standard CP (19%) diet (positive control; PC) and a negative control (NC) diet with low CP (16%) and reduced Ile, Leu, and histidine levels. The rest of the treatments had low CP with varied EAA types and levels; T1 had similar Ile, Leu, and His levels as PC but with low CP (16%), while T2 had low CP and 10% higher His, Thr, Trp, and Met+Cys compared to PC. The T3 was a low CP diet with 10% supplemental Leu, Ile, and Val compared to PC, while T4 was a low CP diet with 10% supplementation with all the EAA except Lys compared to PC. The initial body weight (BW) was not statistically different (P > 0.05) among the treatments. Also, on d 6, no statistical differences in BW were observed among the treatments. The average BW recorded on d 13, 20, and 27 showed significant treatment differences where the PC had consistently higher BW than all the other treatments (P < 0.05). The average daily gain (ADG) of the PC was higher than the rest of the treatments. Between d 13 and 20, the average daily feed intake (ADFI) for PC was not different from NC and T1 (P > 0.05), but compared to T2, T3, and T4, the PC treatment showed a high ADFI (P < 0.05). Overall (d 0-27), the ADFI for PC was not different from T1 and was significantly higher than all other treatments. Overall, results showed that the gain to feed (G:F) ratio was higher (P < 0.05) for PC compared to other dietary treatments. In summary, although the treatments (T1-T4) consisted of varying levels of EAA above the recommended requirement levels for optimal performance, we did not see a significant impact on growth performance improvement, which may indicate that the targeted EAA (His, Val, Thr, lle, Leu, Trp, and Met) may not have been limiting in these diets. On the other hand, the phenylalanine (Phe) requirement may be limited in the current formulations, or perhaps the EAA: total N ratio in T1, T2, T3, and T4 may have been too high, resulting in the inefficiency of EAA utilization for growth.

O-Methylisourea Can React with the α-Amino Group of Lysine: Implications for the Analysis of Reactive Lysine.

The specificity of O-methylisourea (OMIU) to bind to the ε-amino group of Lys, an important supposition for the OMIU-reactive Lys analysis of foods, feeds, ingredients, and digesta, was investigated. Crystalline l-Lys incubated under standard conditions with OMIU resulted in low homoarginine recoveries. The reaction of OMIU with the α-amino group of Lys was confirmed by MS analysis, with double derivatized Lys being identified. None of the changes in reaction conditions (OMIU pH, OMIU to Lys ratio, and reaction time) with crystalline l-Lys resulted in 100% recovery of homoarginine. The average free Lys content in ileal digesta of growing pigs and broilers was found to be 13% of total Lys, which could result in a significant underestimation of the reactive Lys content. The reaction of OMIU with α-amino groups may necessitate analysis of free Lys to accurately quantify reactive lysine in samples containing a large proportion of Lys with a free α-amino group.